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- Title
The application of a lateral flow immunographic assay to rapidly test for dexamethasone in commercial facial masks.
- Authors
Wang, Min; Guo, Liqun; Yu, Miao; Zhao, Hua
- Abstract
Dexamethasone (DE) is a synthetic glucocorticoid that is frequently added to cosmetic products for its good short-term effects, especially in facial masks, but long-term use is hazardous to the health. The abuse of DE in whitening and acne cosmetic products is currently a serious problem in China. It is necessary to establish a rapid method of detecting illegal DE addition in cosmetics. In the present study, a monoclonal antibody (mAb) against DE, 2D5-3D12, was developed that displayed cross-reactivities of 124.5%, 38.8%, 6.7%, 0.9%, 1.1%, 1.82%, and 2.39% with prednisolone, betamethasone, prednisone, beclomethasone, hydrocortisone, triamcinolone, and flumetasone, respectively. A colloidal gold-based lateral flow immunographic assay based on mAb 2D5-3D12 was established and used to determine the DE contents of commercial facial masks. The indicator range of the immunographic assay for DE was 100–200 ng/mL, and the results were consistent with those afforded by LC-MS. This novel method provides the advantages of simple sample treatment, a user-friendly procedure, and rapid detection.
- Subjects
CHINA; MONOCLONAL antibodies; GLUCOCORTICOIDS; BECLOMETHASONE dipropionate; DEXAMETHASONE
- Publication
Analytical & Bioanalytical Chemistry, 2019, Vol 411, Issue 22, p5703
- ISSN
1618-2642
- Publication type
Academic Journal
- DOI
10.1007/s00216-019-01948-2