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- Title
Cyclic peptide: a safe and effective alternative to synthetic aflatoxin B<sub>1</sub>-competitive antigens.
- Authors
Mukhtar, Hina; Ma, Lan; Pang, Qian; Zhou, Youxiang; Wang, Xiaohong; Xu, Ting; Hammock, Bruce D.; Wang, Jia
- Abstract
Aflatoxin B1 (AFB1) is one of the major mycotoxins, which naturally occurs in food and agricultural products. In this study, a cyclic peptide (CVPSKPGLC) mimicking AFB1 was used to develop a biotinylated peptide–based immunoassay (bp-ELISA) for AFB1 determination. This cyclic peptide was isolated from a commercially available phage-displayed random 7-mer cyclic peptide library, and then synthesized chemically. Instead of phage particles, the peptide was biotinylated and used to detect AFB1 by bp-ELISA, with an IC50 of 0.92 ng/mL, which was approximately 60-fold better than that of phage ELISA. Good recoveries (83–102%) were obtained in spiked rice and corn samples, which were further validated by high-performance liquid chromatography-fluorescence detector. As better sensitivities (0.92–1.21 ng/mL) were obtained by bp-ELISA even using selected anti-AFB1 antibodies prepared previously in laboratory, this cyclic peptide is suitable as a substitute for synthetic competitive AFB1 antigens in food contamination monitoring.
- Subjects
AFLATOXINS; ANTIGENS; FOOD contamination; IMMUNOASSAY; MYCOTOXINS
- Publication
Analytical & Bioanalytical Chemistry, 2019, Vol 411, Issue 17, p3881
- ISSN
1618-2642
- Publication type
Academic Journal
- DOI
10.1007/s00216-019-01862-7