Title: Development of a high sensitivity quantum dot-based fluorescent quenching lateral flow assay for the detection of zearalenone.
Authors: Chen, Yuan; Fu, Qiangqiang; Xie, Jun; Wang, Hong; Tang, Yong
Abstract: Zearalenone (ZEN) is a common carcinogenic toxin related to cereal contamination. In this study, we developed a high sensitivity quantum dot (QD)-based fluorescent quenching lateral flow assay (LFA) for sensitive ZEN detection. The linear detection range of the fluorescent quenching LFA for ZEN was 0.78–25 ng/mL, and the limit of detection was 0.58 ng/mL. In addition, the fluorescent quenching LFA showed high recovery (83.1–93.6%) for detection of ZEN concentrations spiked into corn samples. These results indicate that the QD-based fluorescent quenching LFA may be a valuable tool for preliminary screening of ZEN contamination.
Subjects: ZEARALENONE; MYCOTOXINS; QUANTUM dots; IMMUNOSUPPRESSION; WEIGHT loss
Publication: Analytical & Bioanalytical Chemistry, 2019, Vol 411, Issue 10, p2169
ISSN: 1618-2642
Publication type: Academic Journal
DOI: 10.1007/s00216-019-01652-1

Development of a high sensitivity quantum dot-based fluorescent quenching lateral flow assay for the detection of zearalenone.

Chen, Yuan; Fu, Qiangqiang; Xie, Jun; Wang, Hong; Tang, Yong