Colorimetric determination of glutathione in human serum and cell lines by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite.

Wang, Yanying; Liu, Yaqin; Ding, Fang; Zhu, Xiaoyan; Yang, Li; Zou, Ping; Rao, Hanbing; Zhao, Qingbiao; Wang, Xianxiang

In this study, we developed a simple colorimetric approach to detect glutathione (GSH). The proposed approach is based on the ability of CuS-PDA-Au composite material to catalytically oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to ox-TMB to induce a blue color with an absorption peak centered at 652 nm. However, the introduction of GSH can result in a decrease in oxidized TMB; similarly, it can combine with Au nanoparticles (Au NPs) on the surface of CuS-PDA-Au composite material. Both approaches can result in a fading blue color and a reduction of the absorbance at 652 nm. Based on this above, we proposed a technique to detect GSH quantitatively and qualitatively through UV-Vis spectroscopy and naked eye, respectively. This approach demonstrates a low detection limit of 0.42 μM with a broad detection range of 5 × 10−7-1 × 10−4 M with the assistance of UV-Vis spectroscopy. More importantly, this approach is convenient and rapid. This method was successfully applied to GSH detection in human serum and cell lines.A colorimetric approach has been developed by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite for sensitive glutathione detection.

GLUTATHIONE; PEROXIDASE; CANCER cells; CELL lines; GOLD nanoparticles; ABSORPTION

Analytical & Bioanalytical Chemistry, 2018, Vol 410, Issue 20, p4805

1618-2642

Academic Journal

10.1007/s00216-018-1117-4