Results
Title

Estrogen receptor preparation effects on the receptor-DNA interaction by surface plasmon resonance.

Authors

Bayle, Sandrine; Chopineau, Joel; Roig, Benoit; Habauzit, Denis

Abstract

Up to now, several studies have investigated estrogen receptor (ER)-estrogen response element (ERE) interaction using biosensors such as surface plasmon resonance. These strategies have aimed to understand the molecular mechanism of such interaction as well as the effect of the ligand on this interaction. These approaches start to be used to determine the mechanisms of protein/DNA interaction, in particular in the context of drug discovery or environmental applications. However, some physical and biochemical parameters (incubation time, temperature, protease inhibitor cocktail, and bovine serum albumin (BSA)) are not completely described in the literature and could deeply modify the obtained results. This paper aims to focus not only on the preliminary steps of sample preparation such as protein thawing and incubation conditions (time and temperature) but also on the evaluation of protease inhibitor cocktail and BSA effect on the measurement of ER-ERE interactions.

Subjects

ESTROGEN receptors; EFFECT of chemicals on DNA; INCUBATION period (Communicable diseases); SURFACE plasmon resonance; BIOSENSORS; PROTEASE inhibitors

Publication

Analytical & Bioanalytical Chemistry, 2016, Vol 408, Issue 29, p8257

ISSN

1618-2642

Publication type

Academic Journal

DOI

10.1007/s00216-016-9967-0