Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples.

Bocková, Markéta; Chadtová Song, Xue; Gedeonová, Erika; Levová, Kateřina; Kalousová, Marta; Zima, Tomáš; Homola, Jiří

Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men.

BLOOD proteins; FETAL development; SURFACE plasmon resonance; BLOOD serum analysis; CARRIER proteins

Analytical & Bioanalytical Chemistry, 2016, Vol 408, Issue 26, p7265

1618-2642

Academic Journal

10.1007/s00216-016-9664-z