A self-assembling RNA aptamer-based nanoparticle sensor for fluorometric detection of Neomycin B in milk.

Ling, Kai; Jiang, Hongyan; Zhang, Linlin; Li, Yang; Yang, Lu; Qiu, Chen; Li, Fu-Rong

To date, there are few reports regarding the development of RNA aptamer-based biosensors for the detection of small molecules. The possible reason is attributed to the weak nuclease resistance of RNA in biological environments. In this study, we have developed an RNA aptamer-based gold nanoparticle (AuNP) sensor for fluorometric detection of Neomycin B in milk. This aptasensor depends on the self-assembly of the RNA aptamer/Neomycin B complex and fluorescence quenching by AuNPs. This biosensor exhibited a low detection limit of 0.01 μM, with a linear dynamic range from 0.1 to 10 μM in milk, and a good selectivity toward Neomycin B. Specifically, because of the shorter RNA fragments and the nuclease inhibition ability of the RNA-modified AuNPs, the RNA sequences remained stable during the experiments. This work will serve as an example for the development of novel biosensors based on RNA aptamers. [Figure not available: see fulltext.]

RNA; NUCLEIC acids; ANTISENSE RNA; ARCHAEBACTERIA RNA; COMPLEMENTARY RNA

Analytical & Bioanalytical Chemistry, 2016, Vol 408, Issue 13, p3593

1618-2642

Academic Journal

10.1007/s00216-016-9441-z