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- Title
Localization of double bonds in triacylglycerols using high-performance liquid chromatography/atmospheric pressure chemical ionization ion-trap mass spectrometry.
- Authors
Háková, Eva; Vrkoslav, Vladimír; Míková, Radka; Schwarzová-Pecková, Karolina; Bosáková, Zuzana; Cvačka, Josef
- Abstract
A method for localizing double bonds in triacylglycerols using high-performance liquid chromatography-tandem mass spectrometry with atmospheric pressure chemical ionization (APCI) was developed. The technique was based on collision-induced dissociation or pulsed Q collision-induced dissociation of the CHN adducts ([M 55]) formed in the presence of acetonitrile in the APCI source. The spectra were investigated using a large series of standards obtained from commercial sources and prepared by randomization. The fragmentation spectra made it possible to determine (i) the total number of carbons and double bonds in the molecule, (ii) the number of carbons and double bonds in acyls, (iii) the acyl in the sn-2 position on the glycerol backbone, and (iv) the double-bond positions in acyls. The double-bond positions were determined based on two types of fragments (alpha and omega ions) formed by cleavages of C-C bonds vinylic to the original double bond. The composition of the acyls and their positions on glycerol were established from the masses and intensities of the ions formed by the elimination of fatty acids from the [M 55] precursor. The method was applied for the analysis of triacylglycerols in olive oil and vernix caseosa. [Figure not available: see fulltext.]
- Subjects
DOUBLE bonds; TRIGLYCERIDES; LIQUID chromatography; ATMOSPHERIC pressure; CHEMICAL ionization mass spectrometry; CARBON; MOLECULES
- Publication
Analytical & Bioanalytical Chemistry, 2015, Vol 407, Issue 17, p5175
- ISSN
1618-2642
- Publication type
Academic Journal
- DOI
10.1007/s00216-015-8537-1