Facile preparation of organic-inorganic hybrid polymeric ionic liquid monolithic column with a one-pot process for protein separation in capillary electrochromatography.

Liu, Cuicui; Deng, Qiliang; Fang, Guozhen; Feng, Xue; Qian, Hailong; Wang, Shuo

An organic-inorganic hybrid monolithic column based on 1-vinyl-3-dodecylimidazolium bromide (VCImBr) has been prepared in a single step by combining radical copolymerization with a non-hydrolytic sol-gel (NHSG) process. The NHSG process was significantly shortened to 6 h by using formic acid as catalyst. For comparison, we also prepared polymeric ionic liquid (PIL) monolithic columns by hydrolytic sol-gel and organic polymeric process, respectively. The resulting monolithic columns were characterized by Fourier transform infrared spectra, scanning electron microscopy, and Brunauer-Emmett-Teller. Under the capillary electrochromatography mode, these columns were applied to separate alkylbenzenes, anilines, and proteins, respectively. The results indicated that the NHSG-based hybrid PIL monolithic column exhibited the highest column efficiency among the three types of columns; organic solvent, commonly required by the traditional columns to achieve satisfactory separation efficiency for proteins, was absent in the NHSG-based hybrid PIL monolithic column because of the biocompatibility of the VCImBr, which was beneficial to analysis of protein containing samples. In order to demonstrate its application potential, the developed NHSG-based hybrid PIL monolithic column was also employed to separate egg white sample.

CAPILLARY electrochromatography; PROTEIN fractionation; SOL-gel processes; COPOLYMERIZATION; BROMIDES

Analytical & Bioanalytical Chemistry, 2014, Vol 406, Issue 28, p7175

1618-2642

Academic Journal

10.1007/s00216-014-8137-5