Results
Title

Quantitative bioimaging of p-boronophenylalanine in thin liver tissue sections as a tool for treatment planning in boron neutron capture therapy.

Authors

Reifschneider, Olga; Schütz, Christian; Brochhausen, Christoph; Hampel, Gabriele; Ross, Tobias; Sperling, Michael; Karst, Uwe

Abstract

An analytical method using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was developed and applied to assess enrichment of B-containing p-boronophenylalanine-fructose (BPA-f) and its pharmacokinetic distribution in human tissues after application for boron neutron capture therapy (BNCT). High spatial resolution (50 μm) and limits of detection in the low parts-per-billion range were achieved using a Nd:YAG laser of 213 nm wavelength. External calibration by means of B-enriched standards based on whole blood proved to yield precise quantification results. Using this calibration method, quantification of B in cancerous and healthy tissue was carried out. Additionally, the distribution of B was investigated, providing B enrichment in the investigated tissues. Quantitative imaging of B by means of LA-ICP-MS was demonstrated as a new option to characterise the efficacy of boron compounds for BNCT.

Subjects

QUANTITATIVE chemical analysis; BORON-neutron capture therapy; LASER ablation; LIVER cells; INDUCTIVELY coupled plasma mass spectrometry; PHENYLALANINE; MEDICAL imaging systems

Publication

Analytical & Bioanalytical Chemistry, 2015, Vol 407, Issue 9, p2365

ISSN

1618-2642

Publication type

Academic Journal

DOI

10.1007/s00216-014-8012-4