Determination of global DNA methylation level by capillary electrophoresis using octyl-modified quaternized cellulose as an electrolyte additive.

Huang, Dihui; Yang, Qin; Jin, Shanxia; Deng, Qianchun; Zhou, Ping

A new cellulose derivative, octyl-modified quaternized cellulose (OMQC), was synthesized and used as electrolyte additive for the analysis of 5-methylcytosine by capillary electrophoresis with UV detection. While added in the background electrolyte, OMQC carrying octyl groups and quaternary ammonium groups exhibited dynamic coating ability. Capillary coated with OMQC was able to generate a stable anodal electro-osmotic flow even at pH 12.0. After several running conditions were optimized, a new method for quantification of genomic methylation level was developed on the basis of hydrolysis of DNA by formic acid and separation of nucleic acid bases by capillary electrophoresis. Cytosine and 5-methylcytosine were separated with a resolution near 4.0 in less than 10 min. The detection limits (S/N = 3) were 1.1 and 1.5 μg/mL for cytosine and 5-methylcytosine, respectively.

DNA methylation; GENETICS; ELECTROPHORESIS; CELLULOSE; ELECTROLYTES

Analytical & Bioanalytical Chemistry, 2014, Vol 406, Issue 12, p2771

1618-2642

Academic Journal

10.1007/s00216-014-7698-7