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- Title
Micro- to nanostructured poly(pyrrole-nitrilotriacetic acid) films via nanosphere templates: applications to 3D enzyme attachment by affinity interactions.
- Authors
Cernat, Andreea; Le Goff, Alan; Holzinger, Michael; Sandulescu, Robert; Cosnier, Serge
- Abstract
We report the combination of latex nanosphere lithography with electropolymerization of N-substituted pyrrole monomer bearing a nitrilotriacetic acid (NTA) moiety for the template-assisted nanostructuration of poly(pyrrole-NTA) films and their application for biomolecule immobilization. The electrodes were modified by casting latex beads (100 or 900 nm in diameter) on their surface followed by electropolymerization of the pyrrole-NTA monomer and the subsequent chelation of Cu ions. The dissolution of the nanobeads leads then to a nanostructured polymer film with increased surface. Thanks to the versatile affinity interactions between the (NTA)Cu complex and histidine- or biotin-tagged proteins, both tyrosinase and glucose oxidase were immobilized on the modified electrode. Nanostructuration of the polypyrrole via nanosphere lithography (NSL) using 900- and 100-nm latex beads allows an increase in surface concentration of enzymes anchored on the functionalized polypyrrole electrode. The nanostructured enzyme electrodes were characterized by fluorescence microscopy, 3D laser scanning confocal microscopy, and scanning electron microscopy. Electrochemical studies demonstrate the increase in the amount of immobilized biomolecules and associated biosensor performances when achieving NSL compared to conventional polymer formation without bead template. In addition, the decrease in nanobead diameter from 900 to 100 nm provides an enhancement in biosensor performance. Between biosensors based on films polymerized without nanobeads and with 100-nm nanobeads, maximum current density values increase from 4 to 56 μA cm and from 7 to 45 μA cm for biosensors based on tyrosinase and glucose oxidase, respectively.
- Subjects
LITHOGRAPHY; ELECTROPOLYMERIZATION; NITRILOTRIACETIC acid; GLUCOSE oxidase; BIOSENSORS; PHENOL oxidase
- Publication
Analytical & Bioanalytical Chemistry, 2014, Vol 406, Issue 4, p1141
- ISSN
1618-2642
- Publication type
Academic Journal
- DOI
10.1007/s00216-013-7135-3