Metabolomics for unknown plant metabolites.

Nakabayashi, Ryo; Saito, Kazuki

In this article we discuss current trends in the techniques available for plant metabolomics. Chemical assignment of unknown metabolites leads to understanding of biosynthetic mechanisms at the gene level for genome-sequenced plants. Metabolomics using mass spectrometry has achieved innovative results in phytochemical genomics for primary and secondary metabolism in the model plant Arabidopsis thaliana by using publicly and commercially available information and standard compounds. However, finding a consolidated analytical technique for elucidation of structural information (e.g., elemental composition and structure) remains challenging. Recently, hyphenated analytical techniques and computer-assisted structural analysis with high-throughput and high-accuracy have been developing. Metabolite-driven approaches using such technology will be of central importance in phytochemical genomics.

METABOLOMICS; METABOLITES; GENOMICS; PHYTOCHEMICALS; SPECTRUM analysis; TECHNOLOGY

Analytical & Bioanalytical Chemistry, 2013, Vol 405, Issue 15, p5005

1618-2642

Academic Journal

10.1007/s00216-013-6869-2