Development of a microwave-assisted-extraction-based method for the determination of aflatoxins B, G, B, and G in grains and grain products.

Chen, Si; Zhang, Hong

This article describes the use of microwave-assisted extraction (MAE) as a pretreatment technique for the determination of aflatoxins B, G, B, and G in grains and grain products. The optimal operation parameters, including extraction solvent, temperature, and time, were identified to be acetonitrile as the extraction solvent at 80 °C with 15 min of MAE. The extracts were cleaned up using solid-phase extraction followed by derivatization with trifluoroacetic acid and were determined by liquid chromatography-fluorescence detection. A Sep-Pak cartridge was chosen over Oasis HLB and Bond Elut cartridges. By the use of aflatoxin M as an internal standard, relative recoveries of the aflatoxins ranged from 90.7 to 105.7 % for corn and from 88.1 to 103.4 % for wheat, with relative standard deviations between 2.5 and 8.7 %. A total of 36 samples from local markets were analyzed, and aflatoxin B was found to be the predominant toxin, with concentrations ranging from 0.42 to 3.41 μg/kg. [Figure not available: see fulltext.]

AFLATOXINS; MYCOTOXINS; ACETONITRILE; TRIFLUOROACETIC acid; GRAIN

Analytical & Bioanalytical Chemistry, 2013, Vol 405, Issue 5, p1623

1618-2642

Academic Journal

10.1007/s00216-012-6564-8