Results
Title

Microfluidic channel with embedded SERS 2D platform for the aptamer detection of ochratoxin A.

Authors

Galarreta, Betty; Tabatabaei, Mohammadali; Guieu, Valérie; Peyrin, Eric; Lagugné-Labarthet, François

Abstract

A selective aptameric sequence is adsorbed on a two-dimensional nanostructured metallic platform optimized for surface-enhanced Raman spectroscopy (SERS) measurements. Using nanofabrication methods, a metallic nanostructure was prepared by electron-beam lithography onto a glass coverslip surface and embedded within a microfluidic channel made of polydimethylsiloxane, allowing one to monitor in situ SERS fingerprint spectra from the adsorbed molecules on the metallic nanostructures. The gold structure was designed so that its localized surface plasmon resonance matches the excitation wavelength used for the Raman measurement. This optofluidic device is then used to detect the presence of a toxin, namely ochratoxin-A (OTA), in a confined environment, using very small amounts of chemicals, and short data acquisition times, by taking advantage of the optical properties of a SERS platform to magnify the Raman signals of the aptameric monolayer system and avoiding chemical labeling of the aptamer or the OTA target. [Figure not available: see fulltext.]

Subjects

NANOSTRUCTURES; SURFACE enhanced Raman effect; ELECTRON beam lithography; GOLD; SURFACE plasmon resonance; OCHRATOXINS

Publication

Analytical & Bioanalytical Chemistry, 2013, Vol 405, Issue 5, p1613

ISSN

1618-2642

Publication type

Academic Journal

DOI

10.1007/s00216-012-6557-7