We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Recombinant cell bioassays for the detection of (gluco)corticosteroids and endocrine-disrupting potencies of several environmental PCB contaminants.
- Authors
Bovee, Toine; Helsdingen, Richard; Hamers, Astrid; Brouwer, Bram; Nielen, Michel
- Abstract
Sensitive and robust bioassays for glucocorticoids are very useful for the pharmaceutical industry, environmental scientists and veterinary control. Here, a recombinant yeast cell was constructed that expresses the human glucocorticoid receptor alpha and a green fluorescent reporter protein in response to glucocorticoids. Both the receptor construct and the reporter construct were stably integrated into the yeast genome. The correct and specific functioning of this yeast glucocorticoid bioassay was studied by exposures to cortisol and other related compounds and critically compared to a GR-CALUX bioassay based on a human bone cell. Although less sensitive, the new yeast glucocorticoid bioassay showed sensitivity towards all (gluco)corticoids tested, with the following order in relative potencies: budesonide >> corticosterone > dexamethasone > cortisol = betamethasone > prednisolone > aldosterone. Hormone representatives for other hormone nuclear receptors, like 17β-estradiol for the oestrogen receptor, 5α-dihydrotestosterone for the androgen receptor and progesterone for the progesterone receptor, showed no clear agonistic responses, whilst some polychlorinated biphenyls were clearly able to interfere with the GR activity.
- Subjects
GLUCOCORTICOIDS; ANTI-inflammatory agents; PHARMACEUTICAL industry; BIOLOGICAL assay; POLYCHLORINATED biphenyls; GREEN fluorescent protein
- Publication
Analytical & Bioanalytical Chemistry, 2011, Vol 401, Issue 3, p873
- ISSN
1618-2642
- Publication type
Academic Journal
- DOI
10.1007/s00216-011-5162-5