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- Title
Kinetic analysis of the estrogen receptor alpha using RIfS.
- Authors
Fechner, Peter; Pröll, Florian; Albrecht, Christiane; Gauglitz, Günter
- Abstract
The label-free time-resolved reflectometric interference spectroscopy has been used to study the interaction of the human estrogen receptor alpha (ERa) and different types of ligands. Different possible sensor surface coatings including various estrogen derivatives were evaluated for their suitability for detection of ERa. The determination of the kinetic and thermodynamic constants was carried out for the interaction in the heterogeneous phase as well as for the interaction in homogeneous phase. In addition, the affinity of 11 ligands ranging from natural hormones and pharmaceuticals to endocrine disrupting chemicals (EDCs) has been determined with this label-free assay format.
- Subjects
ESTROGEN receptors; CHEMICAL kinetics; STEROID hormones; THERMODYNAMICS; ENDOCRINE disruptors; LIGANDS (Chemistry)
- Publication
Analytical & Bioanalytical Chemistry, 2011, Vol 400, Issue 3, p729
- ISSN
1618-2642
- Publication type
Academic Journal
- DOI
10.1007/s00216-011-4720-1