Title: MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland.
Authors: Szaluś-Jordanow, Olga; Krysztopa-Grzybowska, Katarzyna; Czopowicz, Michał; Moroz, Agata; Mickiewicz, Marcin; Lutyńska, Anna; Kaba, Jarosław; Nalbert, Tomasz; Frymus, Tadeusz
Abstract: Staphylococcus aureus subsp. anaerobius is an etiological agent of Morel’s disease in small ruminants. The infection results in superficial abscesses located near lymph nodes. In the study, molecular analysis based on multilocus sequence typing (MLST) of seven housekeeping genes (arcC, aroE, glp, gmk, pta, tpi, yqiL) and random amplified polymorphic DNA (RAPD) was carried out on 19 S. aureus subsp. anaerobius strains isolated from two different goat herds from Poland. All of the 19 S. aureus subsp. anaerobius strains were found to belong to single MLST and RAPD types which support the high clonality level of this agent. However, the results obtained show clearly that the S. aureus subsp. anaerobius clone found in goats in Poland is different from those previously described. However, it is identical to the ATCC 38844 strain isolated from sheep in Spain, which has not been so far genotyped using MLST.
Subjects: STAPHYLOCOCCUS aureus; GOATS; LYMPH nodes; GENETIC polymorphisms; GENES
Publication: Archives of Microbiology, 2018, Vol 200, Issue 9, p1407
ISSN: 0302-8933
Publication type: Academic Journal
DOI: 10.1007/s00203-018-1568-1

MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland.

Szaluś-Jordanow, Olga; Krysztopa-Grzybowska, Katarzyna; Czopowicz, Michał; Moroz, Agata; Mickiewicz, Marcin; Lutyńska, Anna; Kaba, Jarosław; Nalbert, Tomasz; Frymus, Tadeusz