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- Title
One-step osteochondral repair with cartilage fragments in a composite scaffold.
- Authors
Marmotti, A.; Bruzzone, M.; Bonasia, D.; Castoldi, F.; Rossi, R.; Piras, L.; Maiello, A.; Realmuto, C.; Peretti, G.
- Abstract
Purpose: This study proposes a single-step therapeutic approach for osteochondral defects using autologous cartilage fragments loaded onto a scaffold composed of a hyaluronic acid (HA) derivative, human fibrin glue (FG) and autologous platelet-rich-plasma (PRP), in a rabbit model. The aim is to demonstrate the in vitro outgrowth of chondrocytes from cartilage fragments and the in vivo formation of a functional repair tissue. Methods: In vitro: minced articular cartilage was loaded onto two different types of scaffold (paste or membrane) according to two different HA preparations (injectable HA-derivative or HA-derivative felt). In vivo: trochlear osteochondral defects were created in 50 adult rabbits, which were then assigned to 5 different treatment groups: cartilage fragments loaded onto membrane scaffolds with FG (Group 1) or without FG (Group 2); membrane scaffolds alone with FG (Group 3) or without FG (Group 4); empty defects (Group 5). Membrane scaffolds were used 'in vivo' for simpler preparation and better adhesive properties. Repair processes were evaluated histologically and by immunohistochemistry at 1, 3, and 6 months. Results: An in vitro time-dependent cell outgrowth from cartilage fragments was observed with both types of scaffolds. At 6 months, in vivo, cartilage fragment-loaded scaffolds induced significantly better repair tissue than the scaffold alone using histological scoring. Repair in Group 2 was superior to that in any of the control groups ( p < 0.05). Conclusion: Autologous cartilage fragments loaded onto an HA felt/FG/PRP-scaffold provided an efficient cell source, and allowed for an improvement of the repair process of ostechondral defects in a rabbit model. Human FG, however, hampered the rabbit healing process. These results may have clinical relevance as they show the potential of a novel one-stage repair technique for osteochondral defects.
- Subjects
AUTOLOGOUS chondrocyte implantation; TREATMENT of bone diseases; TISSUE scaffolds; HYALURONIC acid; FIBRIN tissue adhesive; CARTILAGE cells; LABORATORY rabbits
- Publication
Knee Surgery, Sports Traumatology, Arthroscopy, 2012, Vol 20, Issue 12, p2590
- ISSN
0942-2056
- Publication type
Academic Journal
- DOI
10.1007/s00167-012-1920-y