In vitro dopaminergic neuroprotective and in vivo antiparkinsonian-like effects of Δ³,2-hydroxybakuchiol isolated from Psoralea corylifolia (L.).

Zhao, G.; Zheng, X.-W.; Qin, G.-W.; Gai, Y.; Jiang, Z.-H.; Guo, L.-H.

Cocktail recipes containing Psoralea corylifolia seeds (PCS) are used to empirically treat Parkinson disease. A PCS isolate Δ3,2-hydroxybakuchiol (BU) can inhibit dopamine uptake in dopamine transporter (DAT) transfected Chinese hamster ovary (CHO) cells, and dopamine reuptake blockade may provide an alternative approach for ameliorating parkinsonism. Here, we assessed the potential dopaminergic neuroprotective, and antiparkinsonian-like activity of BU. BU sample size was increased by using a scale-up extraction paradigm. Pharmacologically, BU significantly protected SK-N-SH cells from 1-methyl-4-phenylpyridinium (MPP ) insult, produced striking inhibitory actions on dopamine/norepinephrine uptake and WIN35,428 binding in synaptosomes on in vivo administration, and significantly preventing poor performance on rotarod and dopaminergic loss in substantia nigra in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice. BU acts by protecting dopaminergic neurons from MPP injury and preventing against MPTP-induced behavioral and histological lesions in the Parkinson’s disease (PD) model, possibly by inhibiting monoamine transporters. These findings suggest that BU could be meaningful in PD treatment.

PARKINSON'S disease treatment; DOPAMINERGIC mechanisms; DOPAMINE uptake inhibitors; LEGUMES; PSORALEA; COCKTAILS

Cellular & Molecular Life Sciences, 2009, Vol 66, Issue 9, p1617

1420-682X

Academic Journal

10.1007/s00018-009-9030-9