COX-2 mRNA expression decreases in the subplantar muscle of rat paw subjected to carrageenan-induced inflammation after low level laser therapy.

R. Albertini; F. Aimbire; A. Villaverde; J. Silva; M. Costa

Abstract. Objective and design:  Low level laser therapy (LLLT) has been presented as a novel therapy to treat inflammation. Herein we studied the effect of LLLT on the COX-2 mRNA expression in subplantar tissue taken from rats treated with carrageenan. Material:  The groups consisted of 32 rats: A 1 (Saline), A 2 (Carrageenan), A 3 (Carrageenan laser 660 nm) and A 4 (Carrageenan laser 684 nm). Treatment:   A 3 and A 4 were irradiated in the first hour after carrageenan. Methods:  The edema was measured by a plethysmometer and COX-2 mRNA was by RT-PCR. Statistical were evaluated by ANOVA and Tukey-Kramer Test. Results:  Carrageenan increased both edema (A 1= 0.6 0.04 vs. A 2= 2.24 0.08) and COX-2 mRNA (A 1= 1.1 0.26 vs. A 2= 3.52 0.69). Irradiation reduced the edema (A 3= 0.84 0.09; A4= 1.31 0.05) and the COX-2 mRNA (A 3= 2.16 0.28; A 4= 1.86 0.20). Conclusion:  LLLT reduce the expression of COX-2 mRNA.

INFLAMMATORY mediators; MESSENGER RNA; LASERS; THERAPEUTICS

Inflammation Research, 2007, Vol 56, Issue 6, p228

1023-3830

Academic Journal

10.1007/s00011-007-6211-6