Results
Title

A Protocol for High-Throughput Extraction of DNA From Rice Leaves.

Authors

Xin Xu; Kawasaki, Shinji; Fujimura, Tatsuhito; Chuntai Wang

Abstract

Current DNA isolation methods have limitations between speed and purity in high-throughput molecular genetic analysis such as gene mapping and marker-assisted selection programs. We have optimized a simple and rapid method for isolating high-quality genomic DNA from rice that significantly minimizes time and the use of laboratory materials. One person can process as many as 384 samples in 2 h. The isolated DNA is suitable for polymerase chain reaction-based techniques and is stable for no less than 6 mo of storage at 4°C.

Subjects

DNA; GENE mapping; CLONING; GENETIC techniques; GENOMICS

Publication

Plant Molecular Biology Reporter, 2005, Vol 23, Issue 3, p291

ISSN

0735-9640

Publication type

Academic Journal

DOI

10.1007/BF02772759