Characterization of Fusarium Wilt-Resistant and Fusarium Wilt-Susceptible Somaclones of Banana Cultivar Rastali (Musa AAB) by Random Amplified Polymorphic DNA and Retrotransposon Markers.

Muhammad, Asif Javed; Othman, Rofina Yasmin

Two DNA fingerprinting techniques, random amplified polymorphic DNA (RAPD) and inter-retrotransposon amplified polymorphism (IRAP), were used to characterize somaclonal variants of banana. IRAP primers were designed on the basis of repetitive and genome-wide dispersed long terminal repeat (LTR) retrotransposon families for assessing the somaclonal variation in 2 Musa clones resistant and susceptible to Fusarium oxysporum f. sp. cubense race 4. RAPD markers successfully detected genetic variation within and between individuals of the clones. IRAP makers amplified either by a single primer or a combination of primers based on LTR orientation successfully amplified different retrotransposons dispersed in the Musa genome and detected new events of insertions. RAPD markers proved more polymorphic than IRAP markers. Somaclonal variation seems to be the result of numerous indels occurring genome-wide accompanied by the activation of retroelements, as a result of stress caused by micropropagation. It is concluded that characterization of the somaclonal variants requires more than one DNA marker system to detect variation in diverse components of the genome.

FUSARIUM oxysporum; RAPD technique; DNA fingerprinting; FORENSIC genetics techniques; GENOMICS

Plant Molecular Biology Reporter, 2005, Vol 23, Issue 3, p241

0735-9640

Academic Journal

10.1007/BF02772754