NLRP3 inflammasome activation is associated with PM_2.5‐induced cardiac functional and pathological injury in mice.

Duan, Shuyin; Wang, Na; Huang, Li; Zhao, Ying; Shao, Hua; Jin, Yuefei; Zhang, Ruiqin; Li, Chunyang; Wu, Weidong; Wang, Jing; Feng, Feifei

Growing evidences indicate that inflammation induced by PM2.5 exposure has been considered as a major driving force for the development of cardiovascular diseases. However, the mechanisms underlying PM2.5‐induced cardiac injury remain unclear. This study aims to investigate the role of NLRP3 inflammasome in PM2.5‐induced cardiac functional and pathological injury in mice. In this study, BALB/c mice were intratracheally instilled with PM2.5 suspension (4.0 mg/kg BW) for 5 days to set up a cardiac injury model, which was evaluated by electrocardiogram monitoring, HE and Masson staining. Then, the effects of PM2.5 on the expression of α‐SMA, NLRP3, IL‐1β, and IL‐18 proteins and the activation of caspase‐1 and IL‐1β were investigated. The results showed that PM2.5 exposure induced characteristic abnormal ECG changes such as the abnormality of heart rhythm, tachycardia, and T‐wave reduction. Inflammatory cell infiltration and fibrosis were observed in the heart tissues of PM2.5‐exposed mice. Meanwhile, PM2.5 exposure increased the expression of α‐SMA. And, NLRP3 activation‐associated proteins of NLRP3, IL‐1β, IL‐18, Cleaved caspase‐1 p10, and Cleaved IL‐1β were upregulated in heart tissue of PM2.5‐induced mice. In summary, PM2.5 exposure could induce cardiac functional and pathological injury, which may be associated with the activation of NLRP3 inflammasome.

MICE; WOUNDS & injuries; HEART abnormalities; CARDIOVASCULAR development; CARDIOVASCULAR diseases

Environmental Toxicology, 2019, Vol 34, Issue 11, p1246

1520-4081

Academic Journal

10.1002/tox.22825