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- Title
The Role of Sequential Enzyme Treatments on Structural and Physicochemical Properties of Cassava Starch Granules.
- Authors
Guo, Li; Li, Hui; Lu, Lu; Zou, Feixue; Tao, Haiteng; Cui, Bo
- Abstract
To illuminate the relationship between the branch chain structures and physicochemical characteristics of cassava starch and to expand the range of its functionality, cassava starch granules are modified with sequential α‐amylase from hog pancreas, barley β‐amylase, and transglucosidase from Aspergillus niger. The molecular weight and amylose contents of the enzyme‐modified starches decrease, and the modified starches exhibits increased numbers of short and medium chains and a higher branch density compared with those of control starch. The crystal pattern is transformed from A‐type to a mixture of A‐type and V‐type, and the crystallinity is reduced. The enzyme‐modified starches exhibits increased solubility and paste clarity, a more homogeneous crystal distribution, and reduced gelatinization temperatures, gelatinization enthalpy, and viscosity compared with those of control starch. The enzyme‐treated starches exhibit a larger elasticity modulus, a viscous modulus, and a reduced loss angle tangent when the range of the transglucosidase treatment time is from 2 to 4 h, whereas the opposite effect is observed when the transglucosidase treatment time ranges from 8 to 10 h. These findings demonstrate that the rheological properties of the enzyme‐modified starch are significantly dependent on the time of the transglucosidase treatment. Thus, triple enzyme modification may supply a new potential method to obtain the expected hydrophilic, rheological, and thermal properties of cassava starch.
- Subjects
CASSAVA starch; WHEAT starch; DIELECTRIC loss; MOLECULAR weights; THERAPEUTICS; ELASTIC modulus
- Publication
Starch / Staerke, 2019, Vol 71, Issue 7/8, pN.PAG
- ISSN
0038-9056
- Publication type
Academic Journal
- DOI
10.1002/star.201800258