Results
Title

In situ biomimetic formation of nano‐hydroxyapatite crystals on chitosan microspheres.

Authors

Huang, Di; Xu, Mengjie; Niu, Lulu; Pérez, Montserrat; Du, Jingjing; Wei, Yan; Hu, Yinchun; Lian, Xiaojie; Chen, Weiyi

Abstract

Chitosan (CS) is a biocompatible, noncytotoxic biomaterial used before as base material for composites. On the other hand, nano‐hydroxyapatite (nHA) is one of the main components of human bones, highly used for biomedical applications. In this work, CS microspheres were produced under a W/O emulsion system. CS microspheres with calcium ions were then exposed to Na3PO4 solution. In situ biomimetic nHA crystals were formed on CS microspheres to generate 15.14 ± 3.15‐μm composite microspheres. The microspheres were subsequently seeded with MG63 osteoblasts to observe their cell responses. All microspheres were characterized via scanning electron microscopy (SEM), phase‐contrast photomicroscopy, and X‐ray diffraction (XRD) analysis. The results showed flake‐like shape and islet‐like growth of nHA depositions presented on the surface of the CS microspheres. In vitro tests indicated that the CS/nHA microparticles were not only biocompatible but also enhanced cell adhesion and elongation due to the in situ biomimetic synthesis method.

Subjects

BIOMIMETIC materials; BIOMIMETIC synthesis; CHONDROITIN sulfates; CALCIUM ions; SCANNING electron microscopy; CELL adhesion; CRYSTALS

Publication

Polymers for Advanced Technologies, 2020, Vol 31, Issue 1, p36

ISSN

1042-7147

Publication type

Academic Journal

DOI

10.1002/pat.4745