Ceratonia siliqua L. (immature carob bean) inhibits intestinal glucose absorption, improves glucose tolerance and protects against alloxan-induced diabetes in rat.

Rtibi, Kaïs; Selmi, Slimen; Grami, Dhekra; Saidani, Khouloud; Sebai, Hichem; Amri, Mohamed; Eto, Bruno; Marzouki, Lamjed

BACKGROUND This study was designed to investigate the effects of immature carob pod aqueous extract ( ICPAE) on intestinal glucose absorption in vitro and in vivo using an oral glucose tolerance test ( OGTT) as well as the potential antidiabetic effect in alloxan-induced diabetic rats. OGTT was carried by administration of glucose (2 g kg−1, p.o.) and after treatment with extract (50, 100 and 200 mg kg−1 body weight). Diabetes was induced by single intraperitoneal injection of alloxan (150 mg kg−1). However, the extracts at various doses or glibenclamide ( GLB, 10 mg kg−1 body weight) were given by oral administration for 2 weeks. RESULTS ICPAE (50-2000 µg mL−1) exerted dose-dependent reduction of sodium-dependent glucose transport across isolated mice jejunum and the maximal inhibition exceeded 50%.The ICPAE treatment improved glucose tolerance. More importantly, ICPAE at various doses showed a significant reduction in blood glucose and biochemical profiles in diabetic rats. CONCLUSION Our findings confirm that the degree of maturity of carob characterized by a different phytochemical composition may be responsible for these actions. Therefore, these compounds may be used as a food supplement in hyperglycemia and diabetes treatments. © 2016 Society of Chemical Industry

GLUCOSE tolerance tests; ALLOXAN diabetes; ALTERNATIVE treatment for diabetes; GLIBENCLAMIDE; REACTIVE oxygen species; THERAPEUTICS

Journal of the Science of Food & Agriculture, 2017, Vol 97, Issue 8, p2664

0022-5142

Academic Journal

10.1002/jsfa.8091