Results
Title

The changes in miR-130b levels in human serum and the correlation with the severity of diabetic nephropathy.

Authors

Lv, Chuan; Zhou, Yue‐hong; Wu, Can; Shao, Ying; Lu, Can‐lu; Wang, Qiu‐yue

Abstract

Background: Circulating microRNA 130b has been closely associated with multiple diseases in humans such as cancer, obesity and diabetes mellitus. This study evaluates the correlation between serum miR-130b and the severity of diabetic nephropathy evaluated by measurement of albuminuria.Methods: Three hundred twenty-seven patients with type 2 diabetes mellitus (T2DM) were divided into three groups: normoalbuminuria group [diabetes mellitus, urinary albumin to creatinine ratio (UACR) 300 mg/g, n = 68). The levels of serum miR-130b were validated by real-time polymerase chain reaction. Serum transforming growth factor β1 (TGF-β1), hypoxia inducible factor 1α (HIF-1α) and fibronectin were determined by enzyme-linked immunosorbent assay.Results: Compared with control, serum miR-130b levels were significantly decreased in T2DM patients and further decreased in the patients of diabetes mellitus, DN1 and DN2 groups (p Conclusion: Our findings suggest that serum miR-130b may be a new biomarker for the early diagnosis of DN in T2DM. Circulating miR-130b may possibly be involved in the pathological mechanism of DN, such as lipid metabolic disorders, oxidative stress, extracellular matrix deposition and renal fibrosis.

Subjects

TYPE 2 diabetes complications; ALBUMINURIA; CREATININE; DIABETIC nephropathies; ENZYME-linked immunosorbent assay; FIBRONECTINS; GLYCOSYLATED hemoglobin; GROWTH factors; LOW density lipoproteins; TYPE 2 diabetes; POLYMERASE chain reaction; PROTEINS; RNA; TRIGLYCERIDES; CROSS-sectional method; SEVERITY of illness index; BLOOD urea nitrogen

Publication

Diabetes/Metabolism Research & Reviews, 2015, Vol 31, Issue 7, p717

ISSN

1520-7552

Publication type

Academic Journal

DOI

10.1002/dmrr.2659