Results
Title

Preparation and chromatographic performance of chiral peptide‐based stationary phases for enantiomeric separation.

Authors

Ma, Zhengyue; Shang, Panpan; Liu, Delu; Nie, Yangyang; Liu, Yanli; Guo, Xinyuan; Wei, Benben; Bai, Ligai; Qiao, Xiaoqiang

Abstract

This study presents the development of three new chiral stationary phases. They are based on silica modified with peptides containing phenylalanine and proline. Successful analyses and characterizations were conducted using Fourier transform infrared spectra, elemental analysis, and thermogravimetric analysis. After this, the enantioselective performance of the three chiral peptide‐based columns was evaluated. The evaluation used 11 racemic compounds under normal‐phase high performance liquid chromatography mode. Optimized enantiomeric separation conditions were established. Under these conditions, the enantiomers of flurbiprofen and naproxen were successfully separated on CSP‐1 column: the separation factor of these was 1.27 and 1.21, respectively. In addition, the reproducibility of the CSP‐1 column was also investigated. The results of the investigation illustrated that the stationary phases have good reproducibility (RSD = 0.73%, n = 5).

Subjects

CHIRAL stationary phases; HIGH performance liquid chromatography; PHASE separation; RACEMIC mixtures; ELEMENTAL analysis; INFRARED spectra

Publication

Chirality, 2023, Vol 35, Issue 9, p636

ISSN

0899-0042

Publication type

Academic Journal

DOI

10.1002/chir.23564