We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Real‐Time Metabolic Magnetic Resonance Spectroscopy of Pancreatic and Colon Cancer Tumor‐Xenografts with Parahydrogen Hyperpolarized 1‐<sup>13</sup>C Pyruvate‐d<sub>3</sub>.
- Authors
Fries, Lisa M.; Hune, Theresa L. K.; Sternkopf, Sonja; Mamone, Salvatore; Schneider, Kim Lucia; Schulz‐Heddergott, Ramona; Becker, Dorothea; Glöggler, Stefan
- Abstract
Parahydrogen‐induced polarization (PHIP) is an emerging technique to enhance the signal of stable isotope metabolic contrast agents for Magnetic Resonance (MR). The objective of this study is to continue establishing 1‐13C‐pyruvate‐d3, signal‐enhanced via PHIP, as a hyperpolarized contrast agent, obtained in seconds, to monitor metabolism in human cancer. Our focus was on human pancreatic and colon tumor xenografts. 1‐13C‐vinylpyruvate‐d6 was hydrogenated using parahydrogen. Thereafter, the polarization of the protons was transferred to 13C. Following a workup procedure, the free hyperpolarized 1‐13C‐pyruvate‐d3 was obtained in clean aqueous solution. After injection into animals bearing either pancreatic or colon cancer xenografts, slice‐selective MR spectra were acquired and analyzed to determine rate constants of metabolic conversion into lactate and alanine. 1‐13C‐pyruvate‐d3 proved to follow the increased metabolic rate to lactate and alanine in the tumor xenografts.
- Subjects
NUCLEAR magnetic resonance spectroscopy; COLON cancer; CONTRAST media; PANCREATIC cancer; PANCREATIC tumors
- Publication
Chemistry - A European Journal, 2024, Vol 30, Issue 51, p1
- ISSN
0947-6539
- Publication type
Academic Journal
- DOI
10.1002/chem.202400187