Results
Title

Highly Efficient Trityl‐Nitroxide Biradicals for Biomolecular High‐Field Dynamic Nuclear Polarization.

Authors

Cai, Xinyi.; Lucini Paioni, Alessandra; Adler, Agnes; Yao, Ru; Zhang, Wenxiao; Beriashvili, David; Safeer, Adil; Gurinov, Andrei; Rockenbauer, Antal; Song, Yuguang; Baldus, Marc; Liu, Yangping

Abstract

Dynamic nuclear polarization (DNP) is a powerful method to enhance the sensitivity of solid‐state magnetic nuclear resonance (ssNMR) spectroscopy. However, its biomolecular applications at high magnetic fields (preferably>14 T) have so far been limited by the intrinsically low efficiency of polarizing agents and sample preparation aspects. Herein, we report a new class of trityl‐nitroxide biradicals, dubbed SNAPols that combine high DNP efficiency with greatly enhanced hydrophilicity. SNAPol‐1, the best compound in the series, shows DNP enhancement factors at 18.8 T of more than 100 in small molecules and globular proteins and also exhibits strong DNP enhancements in membrane proteins and cellular preparations. By integrating optimal sensitivity and high resolution, we expect widespread applications of this new polarizing agent in high‐field DNP/ssNMR spectroscopy, especially for complex biomolecules.

Subjects

POLARIZATION (Nuclear physics); BIRADICALS; MAGNETIC resonance; GLOBULAR proteins; ELECTRON paramagnetic resonance; MAGNETIC fields; BIOMOLECULES

Publication

Chemistry - A European Journal, 2021, Vol 27, Issue 50, p12758

ISSN

0947-6539

Publication type

Academic Journal

DOI

10.1002/chem.202102253