Results
Title

Attenuation of cannabis withdrawal symptoms by Prosopis farcta extract, its luteolin and melatonin in mice: Involvement of brain‐derived neurotrophic factor and dopamine.

Authors

Ghasemian‐Yadegari, Javad; Adineh, Ahmad; Mohammadi, Hamidreza; Davari, Shima; Veisani, Yousef; Ghaneialvar, Hori; Aidy, Ali; Abbasi, Naser; Karimi, Elahe

Abstract

The aim of this study was the identification of luteolin in Prosopis farcta extract (PFE) and melatonin to evaluate its effect on THC withdrawal syndrome in mice. Luteolin was identified by high‐performance liquid chromatography (HPCL). Signs of toxicity of mice in PFE and luteolin were monitored for LD50 calculation. The behavioral symptoms of THC withdrawal (stereotypies, ambulation, and inactivity time) induced by the rimonabant challenge were illustrated in THC‐dependent mice receiving PFE, luteolin, and melatonin. The expression of mature BDNF (mBDNF) was evaluated by Western blot analysis. The dopamine concentrations were measured using HPLC. PFE and luteolin LD50 were 650 and 220 mg/kg, respectively. PFE (300 mg/kg), all doses of luteolin, and melatonin increased significantly the mBDNF expression and decreased the dopamine concentration. The findings suggest that PFE, luteolin, and melatonin are mighty in reducing the signs of THC withdrawal. It seems these effects were due to a decrease in dopamine concentration level and an increase in mBDNF protein expression in mice brains. Significance statement: Prosopis farcta extract, luteolin, and melatonin are mighty in reducing the signs of THC withdrawal.The mechanism of Prosopis farcta extract, luteolin, and melatonin was due to a decrease in dopamine concentration level and an increase in mBDNF protein expression in mice brains.Inducing, developing, and, measuring addiction to tetrahydrocannabinol in animals has been accompanied by many difficulties.Neurotrophic factors such as NGF were very difficult to measure

Subjects

BRAIN-derived neurotrophic factor; LUTEOLIN; DRUG withdrawal symptoms; MESQUITE; HIGH performance liquid chromatography; COCAINE-induced disorders

Publication

Cell Biochemistry & Function, 2024, Vol 42, Issue 2, p1

ISSN

0263-6484

Publication type

Academic Journal

DOI

10.1002/cbf.3980