Fabrication of a polymer monolithic column via redox system and its application in efficient separation of Chinese herb R heum palmatum L.

Jiang, Xiaoya; Jiao, Xiaoyan; Zhang, Doudou; Bai, Ligai; Liu, Haiyan; Yan, Hongyuan

ABSTRACT A polymer monolithic column was prepared via redox system in a stainless steel column (50 × 4.6 mm i.d.) by using 1,6-hexanediol diacrylate as monomer and ethylene dimethacrylate as cross linking agent. Characteristics of the homemade monolithic column were investigated by scanning electron microscopy, nitrogen adsorption-desorption and mercury intrusion porosimeter, respectively. The results showed that the resulting monolithic column occupied relative uniform porous skeleton structure. Furthermore, the monolith was tested by separating aromatic compounds in conjunction of high performance liquid chromatography with the highest column efficiency of more than 21,000 plate per meter, which was relative high for polymer-based monolithic column with 4.6 mm i.d. At last, the monolithic column was used to simultaneously separate and quantitatively analyzes five active compounds in Chinese herb Rheum palmatum L. The validation of the method was carried out, which indicated that the accuracy represented by recovery was in the range of 98.48% to 101.57%. Limit of detection and limit of quantification for the five active compounds were less than 33.7 ng and 90.8 ng, respectively. All the results indicated that the present method was simple and efficient for quantitative analysis of the five active compounds from Chinese herb Rheum palmatum L. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44874.

TURKEY rhubarb; POLYMERS; MONOLITHIC reactors; OXIDATION-reduction reaction; LIQUID chromatography; SCANNING electron microscopy

Journal of Applied Polymer Science, 2017, Vol 134, Issue 21, pn/a

0021-8995

Academic Journal

10.1002/app.44874