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- Title
An Efficient CRISPR/Cas Cooperative Shearing Platform for Clinical Diagnostics Applications.
- Authors
Zhao, Junhong; Kong, Derong; Zhang, Guanghui; Zhang, Shen; Wu, Yungen; Dai, Changhao; Chen, Yiheng; Yang, Yuetong; Liu, Yunqi; Wei, Dacheng
- Abstract
The CRISPR/Cas system is a powerful genome editing tool and possesses widespread applications in molecular diagnostics, therapeutics and genetic engineering. But easy folding of the target sequences causes remarkable deterioration of the recognition and shear efficiency in the case of single Cas‐CRISPR RNA (crRNA) duplex. Here, we develop a CRISPR/Cas cooperative shearing (CRISPR‐CS) system. Compared with traditional CRISPR/Cas system, two CRISPR/Cas‐crRNA duplexes simultaneously recognize different sites in the target sequence, increasing recognition possibility and shearing efficiency. Cooperative shearing cuts more methylene blue‐ssDNA reporters on the electrode, enabling unamplified nucleic acid electrochemical assay in less than 5 minutes with a detection limit of 9.5×10−20 M, 2 to 9 orders of magnitude lower than those of other electrochemical assays. The CRISPR‐CS platform detects monkeypox, human papilloma virus and amyotrophic lateral sclerosis with an accuracy up to 98.1 %, demonstrating the potential application of the efficient cooperative shearing.
- Subjects
HUMAN papillomavirus; AMYOTROPHIC lateral sclerosis; GENOME editing; GENETIC engineering; NUCLEIC acids
- Publication
Angewandte Chemie, 2024, Vol 136, Issue 52, p1
- ISSN
0044-8249
- Publication type
Academic Journal
- DOI
10.1002/ange.202411705