We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Magnetic Resonance Imaging‐Chemical Exchange Saturation Transfer (MRI‐CEST) Method for the Detection of Water Cycling across Cellular Membranes.
- Authors
Di Gregorio, Enza; Papi, Chiara; Conti, Laura; Di Lorenzo, Antonino; Cavallari, Eleonora; Salvatore, Marco; Cavaliere, Carlo; Ferrauto, Giuseppe; Aime, Silvio
- Abstract
Water cycling across the membrane transporters is considered a hallmark of cellular metabolism and it could be of high diagnostic relevance in the characterization of tumors and other diseases. The method relies on the response of intracellular proton exchanging molecules to the presence of extracellular Gd‐based contrast agents (GBCAs). Paramagnetic GBCAs enhances the relaxation rate of water molecules in the extracellular compartment and, through membrane exchange, the relaxation enhancement is transferred to intracellular molecules. The effect is detected at the MRI‐CEST (Magnetic Resonance Imaging ‐ Chemical Exchange Saturation Transfer) signal of intracellular proton exchanging molecules. The magnitude of the change in the CEST response reports on water cycling across the membrane. The method has been tested on Red Blood Cells and on orthotopic murine models of breast cancer with different degree of malignancy (4T1, TS/A and 168FARN). The distribution of voxels reporting on membrane permeability fits well with the cells' aggressiveness and acts as an early reporter to monitor therapeutic treatments.
- Subjects
MAGNETIZATION transfer; HYDROLOGIC cycle; CELL membranes; MAGNETIC resonance; MAGNETIC resonance imaging
- Publication
Angewandte Chemie, 2024, Vol 136, Issue 6, p1
- ISSN
0044-8249
- Publication type
Academic Journal
- DOI
10.1002/ange.202313485