Title: A Dual‐Metal‐Catalyzed Sequential Cascade Reaction in an Engineered Protein Cage**.
Authors: Ebensperger, Paul; Zmyslia, Mariia; Lohner, Philipp; Braunreuther, Judith; Deuringer, Benedikt; Becherer, Anita; Süss, Regine; Fischer, Anna; Jessen‐Trefzer, Claudia
Abstract: Herein, we describe the creation of an artificial protein cage housing a dual‐metal‐tagged guest protein that catalyzes a linear, two‐step sequential cascade reaction. The guest protein consists of a fusion protein of HaloTag and monomeric rhizavidin. Inside the protein capsid, we established a ruthenium‐catalyzed allylcarbamate deprotection reaction followed by a gold‐catalyzed ring‐closing hydroamination reaction that led to indoles and phenanthridines with an overall yield of up to 66 % in aqueous solutions. Furthermore, we show that the encapsulation stabilizes the metal catalysts against deactivation by air, proteins and cell lysate.
Subjects: SYNTHETIC proteins; CATALYST poisoning; CHIMERIC proteins; METAL catalysts; PROTEINS; AVIDIN
Publication: Angewandte Chemie, 2023, Vol 135, Issue 16, p1
ISSN: 0044-8249
Publication type: Academic Journal
DOI: 10.1002/ange.202218413

A Dual‐Metal‐Catalyzed Sequential Cascade Reaction in an Engineered Protein Cage**.

Ebensperger, Paul; Zmyslia, Mariia; Lohner, Philipp; Braunreuther, Judith; Deuringer, Benedikt; Becherer, Anita; Süss, Regine; Fischer, Anna; Jessen‐Trefzer, Claudia