We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Discovery of a Unique Flavonoid Biosynthesis Mechanism in Fungi by Genome Mining.
- Authors
Zhang, Wei; Zhang, Xuan; Feng, Dandan; Liang, Yajing; Wu, Zhenying; Du, Siyu; Zhou, Yu; Geng, Ce; Men, Ping; Fu, Chunxiang; Huang, Xuenian; Lu, Xuefeng
- Abstract
Flavonoids are important plant natural products with variable structures and bioactivities. All known plant flavonoids are generated under the catalysis of a type III polyketide synthase (PKS) followed by a chalcone isomerase (CHI) and a flavone synthase (FNS). In this study, the biosynthetic gene cluster of chlorflavonin, a fungal flavonoid with acetolactate synthase inhibitory activity, was discovered using a self‐resistance‐gene‐directed strategy. A novel flavonoid biosynthetic pathway in fungi was revealed. A core nonribosomal peptide synthetase‐polyketide synthase (NRPS‐PKS) is responsible for the generation of the key precursor chalcone. Then, a new type of CHI catalyzes the conversion of a chalcone into a flavanone by a histidine‐mediated oxa‐Michael addition mechanism. Finally, the desaturation of flavanone to flavone is catalyzed by a new type of FNS, a flavin mononucleotide (FMN)‐dependent oxidoreductase.
- Subjects
BIOSYNTHESIS; FLAVIN mononucleotide; ACETOLACTATE synthase; FLAVONOIDS; PEPTIDES; PLANT products; CHALCONE
- Publication
Angewandte Chemie, 2023, Vol 135, Issue 12, p1
- ISSN
0044-8249
- Publication type
Academic Journal
- DOI
10.1002/ange.202215529