We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Logical Analysis of Multiple Single‐Nucleotide‐Polymorphisms with Programmable DNA Molecular Computation for Clinical Diagnostics.
- Authors
Zhang, Chao; Zheng, Tingting; Ma, Qian; Yang, Linlin; Zhang, Mingzhi; Wang, Junyan; Teng, Xiaoyan; Miao, Yanyan; Lin, Hsiao‐chu; Yang, Yang; Han, Da
- Abstract
Analyzing complex single‐nucleotide‐polymorphism (SNP) combinations in the genome is important for research and clinical applications, given that different SNP combinations can generate different phenotypic consequences. Recent works have shown that DNA‐based molecular computing is powerful for simultaneously sensing and analyzing complex molecular information. Here, we designed a switching circuit‐based DNA computational scheme that can integrate the sensing of multiple SNPs and simultaneously perform logical analysis of the detected SNP information to directly report clinical outcomes. As a demonstration, we successfully achieved automatic and accurate identification of 21 different blood group genotypes from 83 clinical blood samples with 100 % accuracy compared to sequencing data in a more rapid manner (3 hours). Our method enables a new mode of automatic and logical sensing and analyzing subtle molecular information for clinical diagnosis, as well as guiding personalized medication.
- Subjects
DNA; BLOOD groups; MOLECULAR pathology; SINGLE nucleotide polymorphisms; AUTOMATIC identification; BLOOD group antigens; BLOOD sampling
- Publication
Angewandte Chemie, 2022, Vol 134, Issue 15, p1
- ISSN
0044-8249
- Publication type
Academic Journal
- DOI
10.1002/ange.202117658