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- Title
Evolution of Molecularly Imprinted Enzyme Inhibitors: From Simple Activity Inhibition to Pathological Cell Regulation.
- Authors
Xu, Jingjing; Miao, Haohan; Zou, Lihua; Tse Sum Bui, Bernadette; Haupt, Karsten; Pan, Guoqing
- Abstract
Molecular imprinting represents one of the most promising strategies to design artificial enzyme inhibitors. However, the study of molecularly imprinted enzyme inhibitors (MIEIs) remains at a primary stage. Advanced applications of MIEIs for cell regulation have rarely been explored. Using a solid‐phase oriented imprinting strategy so as to leave the active site of the enzymes accessible, we synthesized two MIEIs that exhibit high specificity and potent inhibitory effects (inhibition constant at low nM range) towards trypsin and angiogenin. The trypsin MIEI inhibits trypsin activity, tryptic digestion‐induced extracellular matrix lysis and cell membrane destruction, indicating its utility in the treatment of active trypsin‐dependent cell injury. The angiogenin MIEI blocks cancer cell proliferation by suppressing the ribonuclease activity of angiogenin and decreasing the angiogenin level inside and outside HeLa cells. Our work demonstrates the versatility of MIEIs for both enzyme inhibition and cell fate manipulation, showing their great potential as therapeutic drugs in biomedicine.
- Subjects
ENZYME inhibitors; CELLULAR control mechanisms; SYNTHETIC enzymes; BINDING sites; CANCER cell proliferation; MOLECULAR imprinting; HELA cells
- Publication
Angewandte Chemie, 2021, Vol 133, Issue 46, p24731
- ISSN
0044-8249
- Publication type
Academic Journal
- DOI
10.1002/ange.202106657