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- Title
Cellular Synthesis and X‐ray Crystal Structure of a Designed Protein Heterocatenane.
- Authors
Liu, Yajie; Duan, Zelin; Fang, Jing; Zhang, Fan; Xiao, Junyu; Zhang, Wen‐Bin
- Abstract
Herein, we report the biosynthesis of protein heterocatenanes using a programmed sequence of multiple post‐translational processing events including intramolecular chain entanglement, in situ backbone cleavage, and spontaneous cyclization. The approach is general, autonomous, and can obviate the need for any additional enzymes. The catenane topology was convincingly proven using a combination of SDS‐PAGE, LC‐MS, size exclusion chromatography, controlled proteolytic digestion, and protein crystallography. The X‐ray crystal structure clearly shows two mechanically interlocked protein rings with intact folded domains. It opens new avenues in the nascent field of protein‐topology engineering.
- Subjects
PROTEIN structure; CRYSTAL structure; GEL permeation chromatography; X-rays; PROTEIN synthesis; LIQUID chromatography-mass spectrometry
- Publication
Angewandte Chemie, 2020, Vol 132, Issue 37, p16256
- ISSN
0044-8249
- Publication type
Academic Journal
- DOI
10.1002/ange.202005490