Title: Ultra‐fast Cycling for Multiplexed Cellular Fluorescence Imaging.
Authors: Ko, Jina; Oh, Juhyun; Ahmed, Maaz S.; Carlson, Jonathan C. T.; Weissleder, Ralph
Abstract: Rapid analysis of single and scant cell populations is essential in modern diagnostics, yet existing methods are often limited and slow. Herein, we describe an ultra‐fast, highly efficient cycling method for the analysis of single cells based on unique linkers for tetrazine (Tz)/trans‐cyclooctene (TCO)‐mediated quenching. Surprisingly, the quenching reaction rates were more than 3 orders of magnitude faster (t1/2 <1 s) than predicted. This allowed multi‐cycle staining and immune cell profiling within an hour, leveraging the accelerated kinetics to open new diagnostic possibilities for rapid cellular analyses.
Subjects: CELL imaging; CELL analysis; CELL populations; MAGNITUDE (Mathematics); CYCLING competitions
Publication: Angewandte Chemie, 2020, Vol 132, Issue 17, p6906
ISSN: 0044-8249
Publication type: Academic Journal
DOI: 10.1002/ange.201915153

Ultra‐fast Cycling for Multiplexed Cellular Fluorescence Imaging.

Ko, Jina; Oh, Juhyun; Ahmed, Maaz S.; Carlson, Jonathan C. T.; Weissleder, Ralph