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- Title
A Native Ternary Complex Trapped in a Crystal Reveals the Catalytic Mechanism of a Retaining Glycosyltransferase.
- Authors
Albesa ‐ Jové, David; Mendoza, Fernanda; Rodrigo ‐ Unzueta, Ane; Gomollón ‐ Bel, Fernando; Cifuente, Javier O.; Urresti, Saioa; Comino, Natalia; Gómez, Hansel; Romero ‐ García, Javier; Lluch, José M.; Sancho ‐ Vaello, Enea; Biarnés, Xevi; Planas, Antoni; Merino, Pedro; Masgrau, Laura; Guerin, Marcelo E.
- Abstract
Glycosyltransferases (GTs) comprise a prominent family of enzymes that play critical roles in a variety of cellular processes, including cell signaling, cell development, and host-pathogen interactions. Glycosyl transfer can proceed with either inversion or retention of the anomeric configuration with respect to the reaction substrates and products. The elucidation of the catalytic mechanism of retaining GTs remains a major challenge. A native ternary complex of a GT in a productive mode for catalysis is reported, that of the retaining glucosyl-3-phosphoglycerate synthase GpgS from M. tuberculosis in the presence of the sugar donor UDP-Glc, the acceptor substrate phosphoglycerate, and the divalent cation cofactor. Through a combination of structural, chemical, enzymatic, molecular dynamics, and quantum-mechanics/molecular-mechanics (QM/MM) calculations, the catalytic mechanism was unraveled, thereby providing a strong experimental support for a front-side substrate-assisted SNi-type reaction.
- Subjects
GLYCOSYLTRANSFERASES; HOST-parasite relationships; CATALYSIS; QUANTUM mechanics; MOLECULAR dynamics
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 34, p10036
- ISSN
0044-8249
- Publication type
Academic Journal
- DOI
10.1002/ange.201504617