Layer‐by‐Layer Deposition of a Polycationic Bottlebrush Polymer with Hyaluronic Acid Reveals Unusual Assembly Mechanism and Selective Effect on Cell Adhesion and Fate.

Wang, Chang‐Sheng; Pham, Duy Anh; Zhang, Hu; Rabanel, Jean‐Michel; Hassanpour, Nahid; Banquy, Xavier

This study provides a detailed molecular mechanism of the layer‐by‐layer (LbL) film formation process involving BB polymers. The roles of adsorbed and non‐adsorbed chain segments during the layer buildup are revealed by employing surface force apparatus (SFA) and atomic force microscopy (AFM) to probe critical aspects such as surface coverage, polymer conformation at the surface, adhesive properties, and morphology after each layer deposition. The results show that the thickness, nanomechanical properties, and surface chemistry of the nanofilms are significantly influenced by the number of layers. All these cues have an important impact on cell behavior when employed as coatings for culture substrates. The evidence is shown that cells respond very differently to these cues and exhibit behavior that goes from superproliferation down to accelerated death.

ATOMIC force microscopy; SURFACE forces; HYALURONIC acid; SUBSTRATES (Materials science); NANOFILMS

Advanced Functional Materials, 2024, Vol 34, Issue 38, p1

1616-301X

Academic Journal

10.1002/adfm.202402960