Title: Synthetic Biology: Flipping the Switch on Clathrin-Mediated Endocytosis using Thermally Responsive Protein Microdomains (Adv. Funct. Mater. 34/2014).
Authors: Pastuszka, Martha K.; Okamoto, Curtis T.; Hamm‐Alvarez, Sarah F.; MacKay, J. Andrew
Abstract: Elastin‐like polypeptides can be expressed in eukaryotic cells in fusion with functional proteins. When heated, they assemble organelle‐sized microdomains that sequester target complexes. On page 5340, J. A. MacKay and team demonstrate that, by fusion to clathrin‐light chain, these microdomains co‐localize and sequester clathrin heavy chain, which is necessary for clathrin‐mediated endocytosis. Assembly rapidly and reversibly blocks internalization of G‐protein coupled receptors. Image created by Isaac Mora.
Subjects: PERIODICALS; MATERIALS science periodicals
Publication: Advanced Functional Materials, 2014, Vol 24, Issue 34, p5309
ISSN: 1616-301X
Publication type: Academic Journal
DOI: 10.1002/adfm.201470224

Synthetic Biology: Flipping the Switch on Clathrin-Mediated Endocytosis using Thermally Responsive Protein Microdomains (Adv. Funct. Mater. 34/2014).

Pastuszka, Martha K.; Okamoto, Curtis T.; Hamm‐Alvarez, Sarah F.; MacKay, J. Andrew