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- Title
Microspore embryogenesis in Apiaceae.
- Authors
Ferrie, A.; Bethune, T.; Mykytyshyn, M.
- Abstract
Haploid technology is used to develop uniform, true-breeding lines, as well as to accelerate crop improvement programs. Among 20 Apiaceae species screened for response to doubled haploidy, 11 generated microspore-derived embryos, and all but one of the latter yielded doubled haploid plants. Donor plant conditions, basal media, and culture conditions were evaluated for their efficacy on inducing microspore-derived embryos. Growing donor plants at temperature conditions of 10/5 or 15/10°C promoted microspore embryogenesis in fennel ( Foeniculum vulgare Mill.), whereas, growing donor plants at a temperature regime of 10/5°C, along with the use of a cold extraction method, enhanced embryogenesis in dill ( Anethum graveolens L.) and anise ( Pimpinella anisum L.). The culture of fennel and dill microspores in an NLN basal medium and caraway ( Carum carvi L.) in AT-3 basal medium promoted the highest frequencies of embryo induction.
- Subjects
MICROSPOREA; PLANT embryology; UMBELLIFERAE; HAPLOIDY; PLANT breeding; CROP improvement; FENNEL; DILL; PLOIDY; PIMPINELLA
- Publication
Plant Cell, Tissue & Organ Culture, 2011, Vol 104, Issue 3, p399
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-010-9770-0