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- Title
Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma Cells.
- Authors
Shuang-ling Li; Jing Yang; Xiao-fei Lei; Jian-na Zhang; Hong-li Yang; Kun Li; Chang-qing Xu
- Abstract
Background/Aims: In the present study, we describe a novel and straightforward approach to produce a cyclic- arginine-glycine-aspartic (RGD)-peptide-conjugated quantum dot (QD) probe as an ideal target tumor biomarker. Due to its specific structure, the probe can be used for targeted imaging of pancreatic carcinoma cells. Methods: Pancreatic carcinoma cells were routinely cultured and marked with QD-RGD probe. The QD-RGD probe on the fluorescencelabeled cancer cell was observed by fluorescence microscopy and laser confocal microscopy. Cancer cell viability was detected by MTT assay after culturing with QD-RGD probe. Results: Fluorescence microscopy and laser confocal microscopy displayed that 10nmol/L QD-RGD probe was able to effectively mark pancreatic carcinoma cells. In comparison with organic dyes and fluorescent proteins, the quantum dot-RGD probe had unique optical and electronic properties. Conclusion: QD-RGD probe has a low cytotoxicity with an excellent optical property and biocompatibility. These findings support further evaluation of QD-RGD probes for the early detection of pancreatic cancer.
- Subjects
PANCREATIC cancer diagnosis; CANCER cells; DIAGNOSTIC imaging; QUANTUM dots; BIOMARKERS; FLUORESCENCE microscopy; FLUORESCENT proteins
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2016, Vol 38, Issue 5, p1121
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000443062