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- Title
Construction and characterization of a normalized cDNA library from the river snail Bellamya aeruginosa after exposure to copper.
- Authors
Li, Zi-Cheng; An, Li-Hui; Fu, Qing; Liu, Ying; Zhang, Lei; Chen, Hao; Zhao, Xing-Ru; Wang, Li-Jing; Zheng, Bing-Hui; Zhang, Lin-Bo
- Abstract
The construction of a normalized cDNA library is a popular tool for identifying novel biomarkers for monitoring environmental pollution. In the present study, a normalized cDNA library was constructed from the river snail Bellamya aeruginosa after exposure to Cu by using the SMART technique. The titer of the cDNA library was 1.78 × 10 pfu/ml, with a recombinant efficiency of 95.8%. In addition, from 6,000 randomly selected and sequenced clones, 5,473 high-quality ESTs were identified. After processing the sequences, 3,961 unigenes representing 897 contigs and 3,064 singlets were obtained with 27.6% redundancy. Analysis of expressed sequenced tags using COG and GO annotation and KEGG pathway data showed that a large group of genes related to growth and development, signal transduction, and defense mechanisms were present in the cDNA library. Based on our findings, this normalized cDNA library will provide a valuable resource for further research on functional genes and ecotoxicology in B. aeruginosa.
- Subjects
SNAILS; COPPER; BIOMARKERS; MOLECULAR cloning; POLLUTION
- Publication
Ecotoxicology, 2012, Vol 21, Issue 1, p260
- ISSN
0963-9292
- Publication type
Article
- DOI
10.1007/s10646-011-0786-y