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- Title
Establishing Standard Protocols for Bacterial Culture in Biological Research in Canisters (BRIC) Hardware.
- Authors
Fajardo-Cavazos, Patricia; Nicholson, Wayne L.
- Abstract
The NASA GeneLab Data System (GLDS) was recently developed to facilitate crossexperiment comparisons in order to understand the response of microorganisms to the human spaceflight environment. However, prior spaceflight experiments have been conducted using a wide variety of different hardware, media, culture conditions, and procedures. Such confounding factors could potentially mask true differences in gene expression between spaceflight and ground control samples. In an attempt to mitigate such confounding factors, we describe here the development of a standardized set of hardware, media, and protocols for liquid cultivation of microbes in Biological Research in Canisters (BRIC) spaceflight hardware, using the model bacteria Bacillus subtilis strain 168 and Staphylococcus aureus strain UAMS-1 as examples.
- Subjects
SPACE flight; BACTERIAL cultures; BACILLUS subtilis; CONFOUNDING variables; BIOLOGICAL research
- Publication
Gravitational & Space Research, 2016, Vol 4, Issue 2, p58
- ISSN
2332-7774
- Publication type
Article
- DOI
10.2478/gsr-2016-0013