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- Title
Coordination between MIDASIN 1-mediated ribosome biogenesis and auxin modulates plant development.
- Authors
Li, Ke; Zhou, Ximeng; Sun, Xueping; Li, Guanghui; Hou, Lei; Zhao, Shuzhen; Zhao, Chuanzhi; Ma, Changle; Li, Pengcheng; Wang, Xingjun
- Abstract
Ribosomes are required for plant growth and development, and ribosome biogenesis-deficient mutants generally display auxin-related phenotypes. Although the relationship between ribosome dysfunction and auxin is known, many aspects of this subject remain to be understood. We previously reported that MIDASIN 1 (MDN1) is an essential pre-60S ribosome biogenesis factor (RBF) in Arabidopsis. In this study, we further characterized the aberrant auxin-related phenotypes of mdn1-1 , a weak mutant allele of MDN1. Auxin response is disturbed in both shoots and roots of mdn1-1 , as indicated by the DR5:GUS reporter. By combining transcriptome profiling analysis and reporter gene detection, we found that expression of genes involved in auxin biosynthesis, transport, and signaling is changed in mdn1-1. Furthermore, MDN1 deficiency affects the post-transcriptional regulation and protein distribution of PIN-FORMED 2 (PIN2, an auxin efflux facilitator) in mdn1-1 roots. These results indicate that MDN1 is required for maintaining the auxin system. More interestingly, MDN1 is an auxin-responsive gene, and its promoter can be targeted by multiple AUXIN RESPONSE FACTORs (ARFs), including ARF7 and ARF19, in vitro. Indeed, in arf7 arf19 , the auxin sensitivity of MDN1 expression is significantly reduced. Together, our results reveal a coordination mechanism between auxin and MDN1-dependent ribosome biogenesis for regulating plant development.
- Subjects
ORGANELLE formation; AUXIN; PLANT development; RIBOSOMES; REPORTER genes; PLANT growth
- Publication
Journal of Experimental Botany, 2021, Vol 72, Issue 7, p2501
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erab025