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- Title
Antioxidative system activity as the marker for freezing and drought tolerance in winter barley (Hordeum vulgare L.).
- Authors
Żur, Iwona; Surówka, Ewa; Dubas, Ewa; Nowicka, Anna; Krzewska, Monika; Hura, Tomasz; Rapacz, Marcin; Wójcik-Jagła, Magdalena; Ostrowska, Agnieszka; Malaga, Sabina; Janowiak, Franciszek
- Abstract
Any disturbance in physiological homeostasis of a plant can lead to the overproduction of reactive oxygen species (ROS), often resulting in a cascade of uncontrolled oxidation. For that reason plants evolved a sophisticated defence system, in which the most important role is played by antioxidative enzymes - superoxide dismutase (SOD), catalyse (CAT) and peroxidase (POX) - working in concert with low molecular weight (LMW), non-enzymatic antioxidants to protect plant cells from oxidative damage. In the presented study, the role of the antioxidative system in plant acclimation to low temperature and drought was studied with the use of doubled haploid (DH) lines of winter barley (Hordeum vulgare L.), produced by anther culture method from F1 generation of Polish breeding materials. Ten DH lines selected for the study showed significant variation in respect of freezing and drought tolerance level (Gołebiowska-Pikania et al. 2017a, b). A generally uniform mechanism of plant defence against oxidative stress induced by low temperature and drought was revealed. Analysing the effects induced by freezing tolerance acquisition (20-day cold hardening of seedlings at 4/2°C (day/night)) and drought treatment (14 days at 35% soil water content at the generative phase of development) in selected DH lines of barley, it could be supposed that higher tolerance to both studied stress factors was associated with stable (drought) or increased (freezing) SOD activity. Moreover, although both stress factors significantly decreased CAT activity in all studied DH lines of barley, the amplitude of changes was significantly smaller in the case of more tolerant barley genotypes. In contrast, a greater increase in the activity of POX in cold/drought treated plants was characteristic for less tolerant DH lines of barley, whereas the activity of LMW antioxidants was genotype-dependent without clear relation to stress tolerance level. The results obtained indicate a potential for using the analysed parameters as physiological markers of freezing/drought tolerance level in winter barley.
- Subjects
BARLEY breeding; CROPS; DROUGHT tolerance; EFFECT of cold on crops
- Publication
Cereal Research Communications, 2017, Vol 45, p103
- ISSN
0133-3720
- Publication type
Abstract
- DOI
10.1556/0806.45.2017.100